Decreased CCAAT/enhancer binding protein ß expression inhibits the growth of glioblastoma cells.
Neuroscience
; 176: 110-9, 2011 Mar 10.
Article
en En
| MEDLINE
| ID: mdl-21185356
C/EBPß is a leucine-zipper transcription factor implicated in the control of metabolism, development, cell differentiation, and proliferation. However, it remains unclear its role in tumor development. Here, we show that down-regulation of C/EBPß by RNA interference inhibits proliferation in the GL261 murine glioblastoma cell line, induces an arrest of the cell cycle at the G0/G1 boundary, and diminishes their transformation capacity and migration. In addition, we show that C/EBPß regulates the expression of several DNA damage response- and invasion-related genes. Lastly, C/EBPß depletion significantly retards tumor onset and prolongs survival in a murine orthotopic brain tumor model. Immunohistochemical analysis revealed a significant diminution of proliferating cell nuclear antigen (PCNA) labeling in tumors derived from C/EBPß-depleted GL261 cells compared with that in controls. These results show, for the first time, the dependence of glioma cells on C/EBPß and suggest a potential role of this transcription factor in glioma development.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias Encefálicas
/
Glioblastoma
/
Proteína beta Potenciadora de Unión a CCAAT
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Neuroscience
Año:
2011
Tipo del documento:
Article
País de afiliación:
España
Pais de publicación:
Estados Unidos