Molecular identification of duck and quail common cytokine receptor γ chain genes.
Vet Immunol Immunopathol
; 140(1-2): 159-65, 2011 Mar 15.
Article
en En
| MEDLINE
| ID: mdl-21176852
Common cytokine receptor γ chain (γ(c)) family cytokines play crucial roles in the regulation of innate and adaptive immune responses. Unlike mammals, chickens possess two different γ(c) transcripts. To determine if this difference is present in other avian species, γ(c) cDNA and genomic clones from ducks and quails were investigated. Two different γ(c) transcripts were identified in both species and designated as duck γ(c)-a (duγ(c)-a), duγ(c)-b, quail γ(c)-a (quγ(c)-a), and quγ(c)-b. Comparisons between the duck and quail γ(c) cDNA and genomic sequences indicated that the two transcripts were produced by alternative splicing. Unexpectedly, the duγ(c)-b contained the fifth intron, a frame-switching 88-bp insertion, resulting in a receptor molecule lacking a transmembrane region. These findings indicate a possibility that avian species, unlike mammals, express two different γ(c) transcripts due to alternative splicing. This study is the first demonstration of an alternatively spliced γ(c) isoform that lacks a transmembrane domain.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Análisis de Secuencia de ADN
/
Coturnix
/
Patos
/
Subunidad gamma Común de Receptores de Interleucina
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Vet Immunol Immunopathol
Año:
2011
Tipo del documento:
Article
Pais de publicación:
Países Bajos