Simultaneous calibration of optical tweezers spring constant and position detector response.
Opt Express
; 18(25): 26469-74, 2010 Dec 06.
Article
en En
| MEDLINE
| ID: mdl-21164997
We demonstrate a fast and direct calibration method for systems using a single laser for optical tweezers and particle position detection. The method takes direct advantage of back-focal-plane interferometry measuring not an absolute but a differential position, i.e. the position of the trapped particle relative to the center of the optical tweezers. Therefore, a fast step-wise motion of the optical tweezers yields the impulse response of the trapped particle. Calibration parameters such as the detector's spatial and temporal response and the spring constant of the optical tweezers then follow readily from fitting the measured impulse response.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Algoritmos
/
Pinzas Ópticas
/
Interferometría
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Opt Express
Asunto de la revista:
OFTALMOLOGIA
Año:
2010
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Estados Unidos