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Degree of immobilization of synthetic RGDS(PO(3)H(2))PA peptides on titanium surfaces.
Abe, Yasuhiko; Okazaki, Yohei; Hiasa, Kyou; Hirata, Isao; Yoshida, Yasuhiro; Taji, Tsuyoshi; Suzuki, Kazuomi; Okazaki, Masayuki; Akagawa, Yasumasa.
Afiliación
  • Abe Y; Department of Advanced Prosthodontics, Division of Cervico-Gnathostomatology, Hiroshima University Graduate School of Biomedical Sciences, Japan. abey@hiroshima-u.ac.jp
Dent Mater J ; 29(6): 668-72, 2010 Nov.
Article en En | MEDLINE | ID: mdl-21099158
The purpose of this study was to characterize the chemical interaction between titanium surfaces and the peptide RGDS(PO(3)H(2))PA (P-RGD) synthesized from RGD peptide (RGD) and o-phospho-L-serine (P-Ser), and to determine the degree of peptide immobilization on the titanium surface. X-ray photoelectron spectroscopy showed that the adsorption amount of RGD was significantly smaller than those of P-Ser and P-RGD (p<0.05). Furthermore, although it appeared that P-RGD bonded to the surface, ultrasonic rinsing with water caused it to dissociate, releasing RGD and leaving only S(PO(3)H(2))PA bonded to the surface. These findings show that although it remains difficult to obtain a stable P-RGD layer, the phosphate functional group greatly improves immobilization of the molecule on titanium surfaces.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oligopéptidos / Fosfoserina / Titanio / Fibronectinas / Materiales Dentales / Proteínas Inmovilizadas Límite: Humans Idioma: En Revista: Dent Mater J Año: 2010 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón
Buscar en Google
Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oligopéptidos / Fosfoserina / Titanio / Fibronectinas / Materiales Dentales / Proteínas Inmovilizadas Límite: Humans Idioma: En Revista: Dent Mater J Año: 2010 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón