Stable incorporation of a bacterial gene into adult rat skeletal muscle in vivo.
Am J Physiol
; 258(3 Pt 1): C578-81, 1990 Mar.
Article
en En
| MEDLINE
| ID: mdl-2107753
We have developed a novel technique to incorporate and stably express foreign genes in adult rat skeletal muscle in vivo. Endogeneous satellite cells in skeletal muscle regenerating from bupivacaine damage were infected with an injected retrovirus containing the Escherichia coli beta-galactosidase gene under the promoter control of the Moloney murine leukemia virus long-terminal repeat. Constitutive and stable expression of beta-galactosidase activity was observed in muscle fibers after 6 days and 1 mo of muscle regeneration. Two patterns of expression were observed, diffuse expression within fibers and focal expression associated with the sarcolemma. This technique will allow future experiments with muscle-specific genes and promoters to study the physiological regulation of skeletal muscle gene expression in the intact adult mammal. Furthermore, the technique of stimulating stem cell proliferation to allow retroviral-mediated gene transfer may be generally applicable to other tissues.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Beta-Galactosidasa
/
Galactosidasas
/
Genes Bacterianos
/
Músculos
Límite:
Animals
Idioma:
En
Revista:
Am J Physiol
Año:
1990
Tipo del documento:
Article
Pais de publicación:
Estados Unidos