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Aromatase and 5-alpha reductase gene expression: modulation by pain and morphine treatment in male rats.
Aloisi, Anna Maria; Ceccarelli, Ilaria; Fiorenzani, Paolo; Maddalena, Melinda; Rossi, Alessandra; Tomei, Valentina; Sorda, Giuseppina; Danielli, Barbara; Rovini, Michele; Cappelli, Andrea; Anzini, Maurizio; Giordano, Antonio.
Afiliación
  • Aloisi AM; Department of Physiology, Neuroscience and Applied Physiology Unit, University of Siena, Via Aldo Moro 2, 53100 Siena, Italy. aloisi@unisi.it
Mol Pain ; 6: 69, 2010 Oct 26.
Article en En | MEDLINE | ID: mdl-20977699
BACKGROUND: The steroid hormone testosterone has been found to be greatly reduced by opioids in different experimental and clinical conditions. The purpose of this study on male rats was to determine the effects of a single injection of morphine (5 mg/Kg) on persistent pain (formalin test) and the single or combined effects on p450-aromatase and 5-alpha reductase type 1 mRNA expression in the brain, liver and testis. Testosterone was determined in the plasma and in the brain, morphine was assayed in the plasma. RESULTS: In the morphine-treated rats, there were increases of 5-alpha reductase mRNA expression in the liver and aromatase mRNA expression in the brain and gonads. Morphine was detected in the blood of all morphine-treated rats even though there were no clear analgesic affects in the formalin-treated animals three hours after treatment. Testosterone was greatly reduced in the plasma and brain in morphine-treated subjects. CONCLUSIONS: It appears that morphine administration can induce long-lasting genomic effects in different body areas which contribute to the strong central and peripheral testosterone levels. These changes were not always accompanied by behavioral modifications.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dolor / Aromatasa / Regulación Enzimológica de la Expresión Génica / Colestenona 5 alfa-Reductasa / Morfina Límite: Animals Idioma: En Revista: Mol Pain Asunto de la revista: BIOLOGIA MOLECULAR / NEUROLOGIA / PSICOFISIOLOGIA Año: 2010 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Dolor / Aromatasa / Regulación Enzimológica de la Expresión Génica / Colestenona 5 alfa-Reductasa / Morfina Límite: Animals Idioma: En Revista: Mol Pain Asunto de la revista: BIOLOGIA MOLECULAR / NEUROLOGIA / PSICOFISIOLOGIA Año: 2010 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos