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Inter-alpha-trypsin inhibitor heavy chain 4 as a marker of acute rejection in pancreas allotransplantation in pigs.
García-Gil, F A; Lampreave, F; Fuentes-Broto, L; Carpintero, R; Gonzalvo, E; García, J J; Alvarez-Alegret, R; Alfaro, J; Orden, I; Roda, L.
Afiliación
  • García-Gil FA; Department of Surgery, University of Zaragoza, Hospital Universitario Miguel Servet, Zaragoza, Spain. agarciagil@telefonica.net
Transplant Proc ; 42(8): 3063-9, 2010 Oct.
Article en En | MEDLINE | ID: mdl-20970611
BACKGROUND/AIMS: An early, simple, and reliable marker for acute pancreatic allograft rejection is not available. Inter-alpha-trypsin inhibitor heavy chain 4 (ITIH4) is an interleukin-6-dependent acute-phase positive protein that can act as an anti-inflammatory protein. We studied the response of the ITIH4 in pigs undergoing pancreas allotransplantation (PT) and evaluated this protein as a biomarker for acute graft rejection. METHODS: PT with enteric drainage of the exocrine secretion and systemic venous drainage was performed on 12 Landrace pigs. No immunosuppression was administered. Serum concentrations of glucose, amylase, lipase, insulin, C-peptide, and ITIH4 were determined daily. RESULTS: The response of ITIH4 to PT was early, intense, and prolonged, with 2 peaks in serum concentration. The first peak, which started on day 1 and reached maximum (around 6 mg/dL) on day 3, was attributed to the systemic acute phase response to surgical stress. The second peak, which exceeded the first peak and reached maximum (>8 mg/dL) on day 6, began when the recipients were still normoglycemic, and preceded onset of the diabetic state caused by acute graft rejection by an average of 4 days. CONCLUSION: Serum ITIH4 could help to predict subclinical acute graft rejection after PT in pigs.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Alfa-Globulinas / Biomarcadores / Trasplante de Páncreas / Rechazo de Injerto Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Transplant Proc Año: 2010 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Alfa-Globulinas / Biomarcadores / Trasplante de Páncreas / Rechazo de Injerto Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Transplant Proc Año: 2010 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos