Selective determination of aloin in different matrices by HPTLC densitometry in fluorescence mode.
J Pharm Biomed Anal
; 54(2): 422-5, 2011 Jan 25.
Article
en En
| MEDLINE
| ID: mdl-20933355
A novel method based on the fluorescence excited solely on aloin by a H3BO3 derivatizing procedure, allowed its rapid and selective determination among the co-occurring components in a variety of complex matrices as several Aloes dried extracts and related commercial products. HPTLC LiChrospher silica gel 60 F254S, 20 cm x 10 cm, plates with ethyl formate: CH3OH:H2O (100:14.5:10, v/v) as the mobile phase were used. Densitometric determinations were performed in fluorescence mode, exciting wavelength 365 nm, optical filter K540 after derivatization with H3BO3. The method was validated giving rise to a dependable and high throughput procedure well suited to routine application. Aloin was quantified in the range of 110-330 ng with RSD of repeatability and intermediate precision not exceeding 2.3% and accuracy inside the acceptance limits.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espectrometría de Fluorescencia
/
Cromatografía en Capa Delgada
/
Emodina
Idioma:
En
Revista:
J Pharm Biomed Anal
Año:
2011
Tipo del documento:
Article
País de afiliación:
Italia
Pais de publicación:
Reino Unido