An in vitro method for studying endocrine and exocrine secretion in the perfused isolated rabbit pancreas.
Diabetes Res
; 13(2): 95-100, 1990 Feb.
Article
en En
| MEDLINE
| ID: mdl-2091876
This work describes a perfusion technique adapted to the isolated rabbit pancreas allowing investigation of both the endocrine and exocrine function. The pancreas-duodenum-stomach-spleen complex is removed and perfused with a modified Krebs Ringer Bicarbonate medium. The surgical steps leading to the removal of the complex are described. The endocrine response is studied by measuring insulin release when the pancreas is submitted to successive glucose stimulations and the exocrine function is evaluated by the alpha-chymotrypsin activity of the pancreatic juice harvested during the perfusion.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Páncreas
/
Jugo Pancreático
/
Quimotripsina
/
Islotes Pancreáticos
/
Insulina
Límite:
Animals
Idioma:
En
Revista:
Diabetes Res
Asunto de la revista:
ENDOCRINOLOGIA
Año:
1990
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Reino Unido