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Prevalent genotypes of meticillin-resistant Staphylococcus aureus: report from Pakistan.
Zafar, A; Stone, M; Ibrahim, S; Parveen, Z; Hasan, Z; Khan, E; Hasan, R; Wain, J; Bamford, K.
Afiliación
  • Zafar A; Aga Khan University Hospital, Karachi, Pakistan.
  • Stone M; Department of Microbiology, Imperial College Healthcare NHS Trust, London, UK.
  • Ibrahim S; Aga Khan University Hospital, Karachi, Pakistan.
  • Parveen Z; Aga Khan University Hospital, Karachi, Pakistan.
  • Hasan Z; Aga Khan University Hospital, Karachi, Pakistan.
  • Khan E; Aga Khan University Hospital, Karachi, Pakistan.
  • Hasan R; Aga Khan University Hospital, Karachi, Pakistan.
  • Wain J; Laboratory of Enteric Pathogens, Health Protection Agency, London, UK.
  • Bamford K; Department of Microbiology, Imperial College Healthcare NHS Trust, London, UK.
J Med Microbiol ; 60(Pt 1): 56-62, 2011 Jan.
Article en En | MEDLINE | ID: mdl-20884770
Meticillin-resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen in Pakistan and is emerging in the community. This is one of the first reports of the prevalent genotypes of MRSA in both hospital and community settings in Pakistan. Isolates collected in 2006-2007 were characterized by PFGE, staphylococcal cassette chromosome mec (SCCmec) typing and multilocus sequence typing (MLST). PFGE identified nine pulsotypes, the majority of isolates belonging to pulsotypes A (n=70) and B (n=38), which were predominant among hospital-onset MRSA (HO-MRSA) and community-onset MRSA (CO-MRSA) isolates, respectively. Among the HO-MRSA isolates, variants of SCCmec type III were prevalent, whilst SCCmec type IV or variants were predominant in the CO-MRSA isolates. MLST identified two principal sequence types, ST8 and ST239. An association was observed between ST8, PFGE pulsotype B and SCCmec type IV in the CO-MRSA (ST8-MRSA-IV). Similarly, ST239, PFGE pulsotype A and SCCmec type III were associated with HO-MRSA (ST239-MRSA-III). Therefore, the prevalent genotypes circulating in Pakistan at the time of study were ST8-MRSA-IV and ST239-MRSA-III in the community and hospital settings, respectively. A set of HO-MRSA isolates collected in 1997 were characterized by PFGE and SCCmec typing for comparison. The isolates belonged to two PFGE pulsotypes (A, n=28; B, n=11) and contained just two SCCmec types. These results suggest that an increase in genetic diversity occurred over the period 1997-2007 as a result of either microevolution or the importation of strains from surrounding areas.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones Estafilocócicas / Dermatoglifia del ADN / Técnicas de Tipificación Bacteriana / Staphylococcus aureus Resistente a Meticilina Tipo de estudio: Prevalence_studies / Risk_factors_studies Límite: Humans País/Región como asunto: Asia Idioma: En Revista: J Med Microbiol Año: 2011 Tipo del documento: Article País de afiliación: Pakistán Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones Estafilocócicas / Dermatoglifia del ADN / Técnicas de Tipificación Bacteriana / Staphylococcus aureus Resistente a Meticilina Tipo de estudio: Prevalence_studies / Risk_factors_studies Límite: Humans País/Región como asunto: Asia Idioma: En Revista: J Med Microbiol Año: 2011 Tipo del documento: Article País de afiliación: Pakistán Pais de publicación: Reino Unido