Measurement of diffuse reflectance from combinatorial samples.
Anal Chim Acta
; 677(1): 79-89, 2010 Sep 10.
Article
en En
| MEDLINE
| ID: mdl-20850593
Using a luminescence spectrometer as a platform, a system of fibre-optic probes was created that allows full colour characterisation, fluorescence and phosphorescence spectra to be recorded in diffuse reflectance and in transmission from thick or thin film arrays of combinatorial samples of diameter down to 2 mm and from liquids. An integrating sphere is not required and the method is more versatile than the instrument's fibre-optic plate reader which has conjoined fibre bundles set at a fixed angle. Incident and detected light is routed via separate optical fibre bundles which remain stationary above or below a two-axis table. The validation and calibration are described. A library of 25 members was scanned for both diffuse reflectance (colour) and fluorescence in less than an hour. The method thus combines techniques that conventionally rely on different instruments and makes them amenable for high throughput libraries.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espectrometría de Fluorescencia
Idioma:
En
Revista:
Anal Chim Acta
Año:
2010
Tipo del documento:
Article
Pais de publicación:
Países Bajos