Expression of thrombin and its associated protein in cerebellum of human and rat after intracerebral hemorrhage.
Chin Med J (Engl)
; 123(15): 2077-81, 2010 Aug 05.
Article
en En
| MEDLINE
| ID: mdl-20819545
BACKGROUND: Intracerebral hemorrhage (ICH) can cause brain damage through a number of pathways. The purpose of the study was to explore the effect of thrombin, protease nexin-1 (PN-1) and protease activated receptor-1 (PAR-1) in rat and human cerebellum after ICH. METHODS: A model of ICH was produced in adult Sprague-Dawley rats by direct injection of autologous blood (50 microl) into caudate nucleus. Patients with injured hemorrhage were also enrolled in this study. Different expressions of thrombin, PAR-1, PN-1 were detected in rat and human cerebellum by immunohistochemistry and in situ hybridization. RESULTS: In rat cerebellum, thrombin protein significantly increased at 6 hours and reached the maximum 2 days after ICH. The expression of PAR-1 protein reached the maximum at 24 - 48 hours, and then began to decrease. The expression of PN-1 protein reached the maximum at 3 hours, decreased somewhat after that and increased a little at 5 days after ICH. While in human cerebellum, the changing tendency of thrombin, PAR-1 and PN-1 was almost conform to the rat. CONCLUSION: In cerebellum, thrombin can activate PAR-1 expression after ICH, and PN-1 appears quickly after ICH in order to control the deleterious effect of thrombin.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Trombina
/
Cerebelo
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Hemorragia Cerebral
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Receptor PAR-1
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Serpina E2
Tipo de estudio:
Risk_factors_studies
Límite:
Adult
/
Aged
/
Aged80
/
Animals
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Female
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Humans
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Male
/
Middle aged
Idioma:
En
Revista:
Chin Med J (Engl)
Año:
2010
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
China