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Dilution and magnification effects on image analysis applications in activated sludge characterization.
Mesquita, D P; Dias, O; Elias, R A V; Amaral, A L; Ferreira, E C.
Afiliación
  • Mesquita DP; Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Microsc Microanal ; 16(5): 561-8, 2010 Oct.
Article en En | MEDLINE | ID: mdl-20804639
The properties of activated sludge systems can be characterized using image analysis procedures. When these systems operate with high biomass content, accurate sludge characterization requires samples to be diluted. Selection of the best image acquisition magnification is directly related to the amount of biomass screened. The aim of the present study was to survey the effects of dilution and magnification on the assessment of aggregated and filamentous bacterial content and structure using image analysis procedures. Assessments of biomass content and structure were affected by dilutions. Therefore, the correct operating dilution requires careful consideration. Moreover, the acquisition methodology comprising a 100× magnification allowed data on aggregated and filamentous biomass to be determined and smaller aggregates to be identified and characterized, without affecting the accuracy of lower magnifications regarding biomass representativeness.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aguas del Alcantarillado / Bacterias / Procesamiento de Imagen Asistido por Computador / Microscopía Idioma: En Revista: Microsc Microanal Año: 2010 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aguas del Alcantarillado / Bacterias / Procesamiento de Imagen Asistido por Computador / Microscopía Idioma: En Revista: Microsc Microanal Año: 2010 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Reino Unido