Your browser doesn't support javascript.
loading
Uptake of antitetanus F(ab')2 fragments into eukaryotic cells.
Wellhöner, H H; Bigalke, H; Borcholte, T; Erdmann, G; Eschenhagen, T; Jung, K H; Marxen, P; Peukert, U; Neville, D M; Srinivasachar, K.
Afiliación
  • Wellhöner HH; Department of Toxicology, Medical School of Hannover, FRG.
J Physiol (Paris) ; 84(3): 206-10, 1990.
Article en En | MEDLINE | ID: mdl-2074544
1. In order to introduce antitetanus immunoglobulin fragments into eukaryotic cells, either antitetanus F(ab')2 or Fab' fragments have been linked to carrier molecules. Aciclovir, horseradish peroxidase, wheat germ agglutinin, and transferrin were tried as carriers. 2. F(ab')2-aciclovir and Fab'-horseradish peroxidase were not internalized by NG108-15 neurohybridoma cells. 3. [Fab']2-wheat germ agglutinin and F(ab')2-transferrin conjugates were internalized into various cells. 4. F(ab')2-transferrin conjugates were made with three different linkers: N-succinimidyl 3-(2-pyridyldithio) propionate, bis-maleimido hexane, and bis-maleimidoethoxy propane. All three conjugates were internalized but had a different fate inside the cells.
Asunto(s)
Buscar en Google
Añadir a Mi BVS
Imprimir
XML
PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fragmentos Fab de Inmunoglobulinas / Antitoxina Tetánica / Células Eucariotas Idioma: En Revista: J Physiol (Paris) Asunto de la revista: FISIOLOGIA Año: 1990 Tipo del documento: Article Pais de publicación: Francia
Buscar en Google
Añadir a Mi BVS
Imprimir
XML
PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fragmentos Fab de Inmunoglobulinas / Antitoxina Tetánica / Células Eucariotas Idioma: En Revista: J Physiol (Paris) Asunto de la revista: FISIOLOGIA Año: 1990 Tipo del documento: Article Pais de publicación: Francia