Reversible microbial colonization of germ-free mice reveals the dynamics of IgA immune responses.
Science
; 328(5986): 1705-9, 2010 Jun 25.
Article
en En
| MEDLINE
| ID: mdl-20576892
The lower intestine of adult mammals is densely colonized with nonpathogenic (commensal) microbes. Gut bacteria induce protective immune responses, which ensure host-microbial mutualism. The continuous presence of commensal intestinal bacteria has made it difficult to study mucosal immune dynamics. Here, we report a reversible germ-free colonization system in mice that is independent of diet or antibiotic manipulation. A slow (more than 14 days) onset of a long-lived (half-life over 16 weeks), highly specific anticommensal immunoglobulin A (IgA) response in germ-free mice was observed. Ongoing commensal exposure in colonized mice rapidly abrogated this response. Sequential doses lacked a classical prime-boost effect seen in systemic vaccination, but specific IgA induction occurred as a stepwise response to current bacterial exposure, such that the antibody repertoire matched the existing commensal content.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Inmunoglobulina A
/
Escherichia coli
/
Mucosa Intestinal
/
Anticuerpos Antibacterianos
Límite:
Animals
Idioma:
En
Revista:
Science
Año:
2010
Tipo del documento:
Article
País de afiliación:
Suiza
Pais de publicación:
Estados Unidos