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Nonlinear development of the populations of neurons expressing c-Fos under sustained electrical intracochlear stimulation in the rat auditory brainstem.
Rosskothen-Kuhl, Nicole; Illing, Robert-Benjamin.
Afiliación
  • Rosskothen-Kuhl N; Neurobiological Research Laboratory, Department of Otorhinolaryngology, University of Freiburg, Killianst, 5, D-79106 Freiburg, Germany.
Brain Res ; 1347: 33-41, 2010 Aug 06.
Article en En | MEDLINE | ID: mdl-20570662
The immediate-early-gene c-fos is among the first genes to be expressed following sensory-invoked neuronal activity. Its gene product c-Fos forms the limiting monomer of the heterodimeric activator protein-1 transcription factor that triggers various genes involved in neuroplastic remodeling. This study investigated the pattern of c-Fos expression in anteroventral (AVCN) and dorsal cochlear nucleus (DCN) and central inferior colliculus (CIC) after 45 min, 73 min, 2 h, 3:15 h and 5 h of unilateral electrical intracochlear stimulation (EIS) at 50 Hz in anaesthetized rats. Following EIS, tonotopic c-Fos expression was observed for each stimulation time in ipsilateral AVCN, DCN bilaterally, and contralateral CIC. By counting c-Fos positive nuclei, we discovered temporal non-linearities in the size of the respective population of c-Fos expressing neurons. In all regions investigated, the populations significantly increased from 73 min to 2 h but decreased towards 3:15 h. In AVCN, the number rose again by 5 h of EIS. Remarkably, the same was noted for neurons with large nuclei in deep DCN. In both regions, the population of responsive neurons shifted spatially: In central AVCN, the density of c-Fos positive cells increased significantly from 2 to 5h with medial and lateral regions remaining unchanged. In DCN, the density of large c-Fos positive nuclei fell in the upper and rose in the deep layers from 45 min to 5h of EIS. In conclusion, spatiotemporally varying recruitments of neuronal subpopulations into cellular networks responding to specific patterns of sensory activity take place in the auditory brainstem.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Colículos Inferiores / Regulación de la Expresión Génica / Proteínas Proto-Oncogénicas c-fos / Núcleo Coclear / Plasticidad Neuronal / Neuronas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Brain Res Año: 2010 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Colículos Inferiores / Regulación de la Expresión Génica / Proteínas Proto-Oncogénicas c-fos / Núcleo Coclear / Plasticidad Neuronal / Neuronas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Brain Res Año: 2010 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Países Bajos