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Direct labeling of hMSC with SPIO: the long-term influence on toxicity, chondrogenic differentiation capacity, and intracellular distribution.
Yang, Chung-Yi; Hsiao, Jong-Kai; Tai, Ming-Fong; Chen, Shin-Tai; Cheng, Hui-Ying; Wang, Jaw-Lin; Liu, Hon-Man.
Afiliación
  • Yang CY; Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan.
  • Hsiao JK; Department of Medical Imaging, National Taiwan University Hospital Yun-Lin Branch, Douliu, Taiwan.
  • Tai MF; Department of Medical Imaging, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
  • Chen ST; Department of Medical Imaging, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
  • Cheng HY; Department of Physics, National Tsing Hua University, No. 101 Sec. 2, Kuang Fu Road, Hsinchu, 300, Taiwan.
  • Wang JL; Department of Biochemistry, Musculoskeletal Disease Center, J.L. Pettis VA Medical Center, Loma Linda University, Loma Linda, CA, 92357, USA.
  • Liu HM; Department of Medical Imaging, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
Mol Imaging Biol ; 13(3): 443-451, 2011 Jun.
Article en En | MEDLINE | ID: mdl-20567925
PURPOSE: The purpose of this study was to evaluate the long-term cellular toxicity, labeling efficiency, chondrogenic differentiation capacity, and intracellular distribution following direct superparamagnetic iron oxide (SPIO) nanoparticle labeling of human mesenchymal stem cells (hMSCs) in the absence of transfection agents. PROCEDURES: hMSCs were incubated with a SPIO, Ferucarbotran, at concentrations of 0, 1, 10, and 100 µg Fe/ml for 24 or 72 h. The cell granularity and size change, reactive oxygen species generation, and mitochondria membrane potential were measured by flow cytometry. The differentiation capacity of the cells into chondrocytes was determined by Alcian blue and Safranin-O staining, immunocytochemical analysis, and reverse transcription polymerase chain reaction. RESULTS: The intracellular distribution of the internalized particles was visualized via confocal microscopy. No significant difference was found in the toxicity of labeled cells relative to controls. Successful chondrogenesis of Ferucarbotran-labeled hMSCs was confirmed. The intracellular SPIO nanoparticles were located within the lysosomes. CONCLUSIONS: In conclusion, we have demonstrated the feasibility of direct labeling with Ferucarbotran without impairment of cellular function, toxicity, or inhibition of differentiation capacity. Furthermore, lysosomal metabolism takes place after intracellular uptake of Ferucarbotran.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Coloración y Etiquetado / Diferenciación Celular / Dextranos / Condrogénesis / Espacio Intracelular / Nanopartículas de Magnetita / Células Madre Mesenquimatosas Límite: Humans Idioma: En Revista: Mol Imaging Biol Asunto de la revista: BIOLOGIA MOLECULAR / DIAGNOSTICO POR IMAGEM Año: 2011 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Coloración y Etiquetado / Diferenciación Celular / Dextranos / Condrogénesis / Espacio Intracelular / Nanopartículas de Magnetita / Células Madre Mesenquimatosas Límite: Humans Idioma: En Revista: Mol Imaging Biol Asunto de la revista: BIOLOGIA MOLECULAR / DIAGNOSTICO POR IMAGEM Año: 2011 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Estados Unidos