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Development of a loop-mediated isothermal amplification (LAMP) assay for rapid diagnosis of Babesia canis infections.
Müller, H; Aysul, N; Liu, Z; Salih, D A; Karagenc, T; Beyer, D; Kullmann, B; Ahmed, J S; Seitzer, U.
Afiliación
  • Müller H; Division of Veterinary Infection Biology and Immunology, Research Center Borstel, Borstel, Germany.
Transbound Emerg Dis ; 57(1-2): 63-5, 2010 Apr.
Article en En | MEDLINE | ID: mdl-20537107
Vector-borne diseases are rising in interest due to global warming, which is believed to impact on the distribution of vectors into new areas thus influencing the occurrence and epidemiology of vector-borne pathogens. Babesia canis belongs to the Piroplasmidae and there are three described subspecies, namely B. canis canis, B. canis rossi and B. canis vogeli. They are each transmitted by a different tick-species, Dermacentor reticulatus, Haemaphysalis leachi and Rhipicephalus sanguineus, respectively. There are also differences in the geographical distribution and pathogenicity to dogs of each subspecies. In this study, we aimed to establish a rapid and easy to perform DNA-based test using loop-mediated isothermal amplification to detect all three Babesia canis subspecies in one assay.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Babesia / Babesiosis / Técnicas de Amplificación de Ácido Nucleico / Enfermedades de los Perros Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Transbound Emerg Dis Asunto de la revista: MEDICINA VETERINARIA Año: 2010 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Alemania

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Babesia / Babesiosis / Técnicas de Amplificación de Ácido Nucleico / Enfermedades de los Perros Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Transbound Emerg Dis Asunto de la revista: MEDICINA VETERINARIA Año: 2010 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Alemania