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Atomic force microscopy of hydrated phosphatidylethanolamine bilayers.
Zasadzinski, J A; Helm, C A; Longo, M L; Weisenhorn, A L; Gould, S A; Hansma, P K.
Afiliación
  • Zasadzinski JA; Department of Chemical and Nuclear Engineering, University of California, Santa Barbara 93106.
Biophys J ; 59(3): 755-60, 1991 Mar.
Article en En | MEDLINE | ID: mdl-2049529
We present images of the polar or headgroup regions of bilayers of dimyristoyl-phosphatidylethanolamine (DMPE), deposited by Langmuir-Blodgett deposition onto mica substrates at high surface pressures and imaged under water at room temperature with the optical lever atomic force microscope. The lattice structure of DMPE is visualized with sufficient resolution that the location of individual headgroups can be determined. The forces are sufficiently small that the same area can be repeatedly imaged with a minimum of damage. The DMPE molecules in the bilayer appear to have relatively good long-range orientational order, but rather short-range and poor positional order. These results are in good agreement with x-ray measurements of unsupported lipid monolayers on the water surface, and with electron diffraction of adsorbed monolayers.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfatidiletanolaminas / Membrana Dobles de Lípidos Idioma: En Revista: Biophys J Año: 1991 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fosfatidiletanolaminas / Membrana Dobles de Lípidos Idioma: En Revista: Biophys J Año: 1991 Tipo del documento: Article Pais de publicación: Estados Unidos