Effect of methylglyoxal on glucose formation, drug oxidation and glutathione content in isolated murine hepatocytes.
Biochim Biophys Acta
; 1092(3): 284-90, 1991 May 17.
Article
en En
| MEDLINE
| ID: mdl-2049398
The first stage in the formation of glucose from acetone involves two oxidation steps catalyzed by isozymes of the cytochrome P-450 II E1 gene subfamily; methylglyoxal formed this way is further converted to pyruvate by a reversible conjugation with reduced glutathione. The effect of methylglyoxal on glucose formation, oxidation of aminopyrine, aniline and on reduced glutathione content was investigated in isolated hepatocytes prepared from (i) fasted or (ii) fasted and acetone (known to induce isozymes of P-450 II E1 gene subfamily) pretreated mice. Glucose formation and drug oxidation were increased by methylglyoxal at concentrations below 1 mM, but were severely decreased above 1 mM. Methylglyoxal also decreased protein synthesis at concentrations above 1 mM. If the addition of methylglyoxal was combined with that of other gluconeogenic precursors and glucose the initial increasing effect on drug oxidation was moderated or diminished and the decreasing effect (at high concentrations) was enhanced. The glutathione content of the cells was decreased by methylglyoxal in a concentration dependent manner. Acetone pretreatment of mice also resulted in a decreased glutathione content of the liver. Based on these observations it is assumed that methylglyoxal has contrasting effects in hepatocytes, and can contribute to the disturbed metabolism under circumstances when the acetone production is elevated.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Piruvaldehído
/
Aminopirina
/
Gluconeogénesis
/
Glutatión
/
Hígado
/
Compuestos de Anilina
Límite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1991
Tipo del documento:
Article
País de afiliación:
Hungria
Pais de publicación:
Países Bajos