Detection and identification of Ehrlichia species in Rhipicephalus (Boophilus) microplus ticks in cattle from Xiamen, China.
Vector Borne Zoonotic Dis
; 2011 Jan 18.
Article
en En
| MEDLINE
| ID: mdl-20420536
A total of 289 Rhipicephalus (Boophilus) microplus Canestrini (Acari: Ixodidae) collected from cattle in Xiamen city of Fujian province, southeastern China, were tested for ehrlichial agents by nested polymerase chain reaction assay. Twenty-seven (9.3%) ticks from seven cattle were found positive for Ehrlichia. The polymerase chain reaction products from positive samples were sequenced and found identical with each other. The 1458-bp nucleotide sequence of ehrlichial 16S rRNA gene was obtained and showed the greatest levels of sequence similarity to 16S rRNA gene sequences of the Ehrlichia spp. detected in ticks near Thai-Myanmar-Vietnam border and that in Tibet, China, differing only 2 and 4 bp, respectively. Sequence comparison of the 16S rRNA gene with the members of the genus Ehrlichia reveals that this ehrlichial agent differed from the sequences of E. chaffeensis in ticks from southeastern China by 17 bp, showing close relation to E. chaffeensis. This was the first report on ehrlichial pathogens in R. (Boophilus) microplus from cattle in southeastern China. The spatial distance between Fujian province and Tibet is more, whereas the ehrlichial agents from both regions are highly homologous. The veterinary and public health significances of the agent deserve further investigation.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Vector Borne Zoonotic Dis
Asunto de la revista:
DOENCAS TRANSMISSIVEIS
/
EPIDEMIOLOGIA
Año:
2011
Tipo del documento:
Article
Pais de publicación:
Estados Unidos