A microELISA system for the detection of both anti-HIV-1 and anti-HIV-2 antibodies.
J Immunol Methods
; 139(1): 77-82, 1991 May 17.
Article
en En
| MEDLINE
| ID: mdl-2040818
Current anti-HIV-1 screening tests combine an excellent anti-HIV-1 sensitivity with a sensitivity of only 28-93% for anti-HIV-2 positive plasma or serum samples. The reactivity of anti-HIV-2 sera in anti-HIV-1 screening tests is based mainly on the immunological cross-reactivity of the GAG and POL proteins of HIV-1 and HIV-2. We describe here a sandwich immunoassay, in which HIV-1 viral lysate is combined with an HIV-2 ENV synthetic peptide, corresponding to the immunodominant envelope epitope, as the coating antigens on microELISA plates. This immunoassay has a sensitivity of 100% for anti-HIV-1 (128 sera tested) and 100% for anti-HIV-2 (109 sera tested). Assay specificity with fresh human donor sera was 99.9% (2256 sera tested).
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Anticuerpos Anti-VIH
/
VIH-1
/
VIH-2
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Immunol Methods
Año:
1991
Tipo del documento:
Article
País de afiliación:
Países Bajos
Pais de publicación:
Países Bajos