Tropane alkaloid analysis by chromatographic and electrophoretic techniques: an update.
J Chromatogr B Analyt Technol Biomed Life Sci
; 878(17-18): 1391-406, 2010 May 15.
Article
en En
| MEDLINE
| ID: mdl-20381435
Tropane alkaloids like atropine are antidotes applied against organophosphorus intoxications. Atropine is toxic itself and should be closely monitored during treatment. Hence, simple, fast, and sensitive determination methods for tropane alkaloids in serum are desirable. Mostly adopted methods of analysis are gas chromatography (GC); high performance liquid chromatography (HPLC), and capillary electrophoresis (CE). Various liquid and solid capillary fillings used in micellar electrokinetic chromatography, microemulsion electrokinetic chromatography, capillary electrochromatography, and enantioseparation provide high versatility to CE applications. In HPLC, specialised columns enhance separation efficacy. Ultraviolet light detection is common practise, but recently sensitivity and analyte identification were enhanced by coupling GC, HPLC, and CE to mass spectrometry. Apart from medical treatment, tropane alkaloids, cocaine in particular, are abused with various intentions. Forensic analysis of tropane alkaloids and their metabolites comprises the additional difficulty of unequivocal drug identification. Because of severe legal consequences, sophisticated analytical methods were developed and may provide additional techniques for therapeutic drug monitoring. Examples from forensic cocaine analysis and from doping analysis are included in this review.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espectrometría de Masas
/
Tropanos
/
Cromatografía de Gases
/
Cromatografía Líquida de Alta Presión
/
Electroforesis Capilar
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Chromatogr B Analyt Technol Biomed Life Sci
Asunto de la revista:
ENGENHARIA BIOMEDICA
Año:
2010
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Países Bajos