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Sodium-dependent, concentrative nucleoside transport in Walker 256 rat carcinosarcoma cells.
Crawford, C R; Belt, J A.
Afiliación
  • Crawford CR; Department of Biochemical and Clinical Pharmacology, St. Jude Children's Research Hospital, Memphis, TN 38101.
Biochem Biophys Res Commun ; 175(3): 846-51, 1991 Mar 29.
Article en En | MEDLINE | ID: mdl-2025258
Nucleoside transport in Walker 256 cells was reexamined using formycin B, a nonmetabolized analog of inosine. In the presence of dipyridamole to inhibit the equilibrative (facilitated diffusion) transporter previously described in these cells, the initial rate of uptake of 1 microM formycin B was 10-fold greater in Na(+)-containing medium than in Na(+)-free medium. In the presence of Na+ and dipyridamole the intracellular concentration of formycin B exceeded that in the medium within one min and was 6-fold greater than that of the medium by 5 min. Na(+)-dependent transport of formycin B was inhibited by low concentrations of inosine, but not thymidine. Furthermore, Na(+)-dependent transport of uridine, but not thymidine, was apparent in the presence of dipyridamole. These data indicate that Walker 256 cells have, in addition to the previously described equilibrative transporter, a concentrative nucleoside transporter. The specificity of this transporter appears to correspond to one of the two Na(+)-dependent transporters previously described in mouse intestinal epithelial cells.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sodio / Formicinas / Carcinoma 256 de Walker / Nucleósidos Límite: Animals Idioma: En Revista: Biochem Biophys Res Commun Año: 1991 Tipo del documento: Article Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sodio / Formicinas / Carcinoma 256 de Walker / Nucleósidos Límite: Animals Idioma: En Revista: Biochem Biophys Res Commun Año: 1991 Tipo del documento: Article Pais de publicación: Estados Unidos