Analysis of the protein composition of alfalfa mosaic virus RNA-dependent RNA polymerase.
Virology
; 182(1): 309-15, 1991 May.
Article
en En
| MEDLINE
| ID: mdl-2024468
RNA-dependent RNA polymerase (RdRp) was solubilized and purified from cellular membranes isolated from alfalfa mosaic virus (AIMV)-infected tobacco by employing a procedure recently described for brome mosaic virus RdRp [R. Quadt and E.M.J. Jaspars, 1990, Virology 178, 189-194]. The purified AIMV RdRp is completely dependent on added template RNAs and exhibits a high degree of template specificity. Analysis of the protein composition of AIMV RdRp showed that AIMV-encoded proteins P1 and P2 and the coat protein (CP) are present in the active enzyme complex. Minus-strand synthesis by the AIMV RdRp is inhibited by AIMV CP. Native double-stranded AIMV RNAs are utilized as template for viral RNA synthesis by AIMV RdRp indicating that a helicase activity is present in the purified AIMV RdRp preparation.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ARN Polimerasa Dependiente del ARN
/
Virus del Mosaico
Idioma:
En
Revista:
Virology
Año:
1991
Tipo del documento:
Article
País de afiliación:
Países Bajos
Pais de publicación:
Estados Unidos