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Microbiological quality assessment of laboratory mice in Korea and recommendations for quality improvement.
Na, Yi-Rang; Seok, Seung-Hyeok; Lee, Hui-Young; Baek, Min-Won; Kim, Dong-Jae; Park, Sung-Hoon; Lee, Hyun-Kyoung; Park, Jae-Hak.
Afiliación
  • Na YR; Department of Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University, Seoul, Republic of Korea.
Exp Anim ; 59(1): 25-33, 2010.
Article en En | MEDLINE | ID: mdl-20224167
Regular monitoring of commercial laboratory rodents and institutional research animal residents is essential for microbiological quality control programs. The objective of our study was to investigate the recent prevalence of infectious pathogens in laboratory mice from eight experimental animal vendors and 56 institutional animal facilities in Korea. Our investigation was conducted in 2006-2007. Specific Pathogen Free (SPF) mice from four commercial breeders were clean according to serological, bacteriological, parasitological, and histopathological examination results. However, mice from one intermediate vendor that distributed SPF animals from main commercial vendors to local districts had Syphacia obvelata and Mycoptes musculinus infections. Additionally, mice from conventional animal breeders were highly contaminated. Among the 56 institutional animal facilities, mouse hepatitis virus (MHV), Sendai virus and Mycoplasma pulmonis positive results were obtained in 23.2, 8.9, and 1.8% of animals tested, respectively. These results indicate that quarantine and eradication efforts of infectious pathogens in these facilities are sub-optimal and need to be improved. The use of commercial conventional mice for research should be eliminated and appropriate vendor selection as well as thorough quarantine before releasing animals into a facility are needed. Finally we suggest qualified veterinary experts are needed at each animal facility to ensure an adequate health surveillance program.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Animales de Laboratorio / Ratones Tipo de estudio: Risk_factors_studies Límite: Animals País/Región como asunto: Asia Idioma: En Revista: Exp Anim Asunto de la revista: MEDICINA VETERINARIA Año: 2010 Tipo del documento: Article Pais de publicación: Japón
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Animales de Laboratorio / Ratones Tipo de estudio: Risk_factors_studies Límite: Animals País/Región como asunto: Asia Idioma: En Revista: Exp Anim Asunto de la revista: MEDICINA VETERINARIA Año: 2010 Tipo del documento: Article Pais de publicación: Japón