Evaluation of resistance to Rhabdocline needlecast in Douglas fir variety Shuswap, with quantitative polymerase chain reaction.
Phytopathology
; 100(4): 337-44, 2010 Apr.
Article
en En
| MEDLINE
| ID: mdl-20205537
A quantitative polymerase chain reaction assay was developed that could detect DNA of Rhabdocline pseudotsugae and R. oblonga among DNA of Douglas fir needles to a limit as low as three copies of target DNA. Differential infection rates of two varieties (seed sources) of Douglas fir interplanted in a field were studied in relation to staggered bud breaks. Infection of Douglas fir var. San Isabel corresponded to ascospore release times for Rhabdocline spp., whereas infection of var. Shuswap Lake did not occur throughout the spore release period during 2 years of study, despite abundant inoculum and adequate moisture during bud break. Rhabdocline spp. DNA was never detected in Shuswap Lake and disease symptoms were not observed in any year. We provide evidence that Shuswap Lake is resistant and probably immune to Rhabdocline spp. infection and Rhabdocline needlecast under Michigan conditions.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Enfermedades de las Plantas
/
Ascomicetos
/
Reacción en Cadena de la Polimerasa
/
Pseudotsuga
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Phytopathology
Asunto de la revista:
BOTANICA
Año:
2010
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos