Human retinoblastoma Y79 cells contain both insulin-specific mRNA and insulin-binding sites.

Das, A; Pansky, B; Budd, G C; Reid, T W

Das, A; Pansky, B; Budd, G C; Reid, T W.

Afiliación

Das A; Kresge Eye Institute, Wayne State University, School of Medicine, Detroit, MI 48201.

Neurosci Lett ; 121(1-2): 231-3, 1991 Jan 02.

Article en En | MEDLINE | ID: mdl-2020379

RESUMEN

We previously reported the presence of insulin-like immunoreactivity in cells from the human retinoblastoma Y79 cell line. In the present study, in situ DNA hybridization techniques were applied, using a human insulin cDNA probe to investigate whether the insulin-like activity is due to local synthesis of insulin. Our results suggest that Y79 cells contain mRNA for the synthesis of insulin or a homologous peptide. In addition, 125I-insulin binding autoradiographic studies show that these cells also contain specific insulin-binding sites. It is suggested that insulin may play an autocrine and/or paracrine role in the maintenance and metabolism of the Y79 retinoblastoma cells.

Asunto(s)

Neoplasias del Ojo/metabolismo; Insulina/metabolismo; ARN Mensajero/metabolismo; Receptor de Insulina/metabolismo; Retina; Retinoblastoma/metabolismo; Células Tumorales Cultivadas/metabolismo; Autorradiografía; Humanos; Radioisótopos de Yodo; Hibridación de Ácido Nucleico

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Retina / Retinoblastoma / ARN Mensajero / Receptor de Insulina / Células Tumorales Cultivadas / Neoplasias del Ojo / Insulina Límite: Humans Idioma: En Revista: Neurosci Lett Año: 1991 Tipo del documento: Article Pais de publicación: Irlanda

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