Use of a redox-sensing GFP (c-roGFP1) for real-time monitoring of cytosol redox status in Arabidopsis thaliana water-stressed plants.
FEBS Lett
; 584(5): 889-97, 2010 Mar 05.
Article
en En
| MEDLINE
| ID: mdl-20079738
Using Arabidopsis plants transformed with a redox-sensing green fluorescent protein targeted to the cytosol (c-roGFP1), we have demonstrated, in real time, measurements of reversible changes of redox status in the cytosol of plants subjected to a gradual water-stress, followed by re-watering. Plants sensed water stress, and changed the redox potential of their cytosol to a more oxidized value after a gradually-imposed water stress. Small variations in the cytosol redox potential and ascorbate (AA) values suggest that this parameter was tightly regulated. The re-watering was paralleled by a return of water stress, redox potential and ascorbate to initial values, showing the reversibility of water stress and its consequences.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Arabidopsis
/
Citosol
/
Deshidratación
/
Proteínas Fluorescentes Verdes
Idioma:
En
Revista:
FEBS Lett
Año:
2010
Tipo del documento:
Article
País de afiliación:
España
Pais de publicación:
Reino Unido