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Role of loop 9 on the function of neuronal nicotinic receptors.
Criado, Manuel; Castillo, Mar; Mulet, José; Sala, Francisco; Sala, Salvador.
Afiliación
  • Criado M; Instituto de Neurociencias, Universidad Miguel Hernández-CSIC, Sant Joan d'Alacant, 03550-Alicante, Spain.
Biochim Biophys Acta ; 1798(3): 654-9, 2010 Mar.
Article en En | MEDLINE | ID: mdl-20043866
We have studied the role of loop 9 in the function of neuronal nicotinic receptors. By systematically mutating the residues in the loop we have determined that the most important amino acids determining the coupling of binding to gating are the ones closer to the transmembrane region. Single mutations at location E173 in homomeric alpha7 receptors destroyed their function by completely abolishing the current while preserving the expression at the membrane. In contrast, heteromeric receptor alpha3beta4 with the same mutations retained some function. We conclude that loop 9 has a different role in the function of homomeric and heteromeric receptors.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptores Nicotínicos / Neuronas Límite: Animals / Humans Idioma: En Revista: Biochim Biophys Acta Año: 2010 Tipo del documento: Article País de afiliación: España Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Receptores Nicotínicos / Neuronas Límite: Animals / Humans Idioma: En Revista: Biochim Biophys Acta Año: 2010 Tipo del documento: Article País de afiliación: España Pais de publicación: Países Bajos