Isolation of osteosarcoma-associated human antibodies from a combinatorial Fab phage display library.
J Biomed Biotechnol
; 2009: 157531, 2009.
Article
en En
| MEDLINE
| ID: mdl-20037728
Osteosarcoma, a highly malignant disease, is the most common primary bone tumor and is frequently found in children and adolescents. In order to isolate antibodies against osteosarcoma antigens, a combinatorial osteosarcoma Fab library displayed on the surface of phages was used. After three rounds of selection on the surface of tumor cells, several osteosarcoma-reactive Fabs were detected. From these Fabs, five were better characterized, and despite having differences in their VH (heavy chain variable domain) and Vkappa (kappa chain variable domain) regions, they all bound to a protein with the same molecular mass. Further analysis by cell ELISA and immunocytochemistry suggested that the Fabs recognize a membrane-associated tumor antigen expressed in higher amounts in neoplasic cells than in normal tissue. These results suggest that the human Fabs selected in this work are a valuable tool for the study of this neoplasia.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fragmentos Fab de Inmunoglobulinas
/
Osteosarcoma
/
Biblioteca de Péptidos
/
Anticuerpos Antineoplásicos
Tipo de estudio:
Risk_factors_studies
Límite:
Humans
Idioma:
En
Revista:
J Biomed Biotechnol
Asunto de la revista:
BIOTECNOLOGIA
/
MEDICINA
Año:
2009
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Estados Unidos