Transduction of RNA-directed DNA methylation signals to repressive histone marks in Arabidopsis thaliana.
EMBO J
; 29(2): 352-62, 2010 Jan 20.
Article
en En
| MEDLINE
| ID: mdl-20010696
RNA-directed modification of histones is essential for the maintenance of heterochromatin in higher eukaryotes. In plants, cytosine methylation is an additional factor regulating inactive chromatin, but the mechanisms regulating the coexistence of cytosine methylation and repressive histone modification remain obscure. In this study, we analysed the mechanism of gene silencing mediated by MORPHEUS' MOLECULE1 (MOM1) of Arabidopsis thaliana. Transcript profiling revealed that the majority of up-regulated loci in mom1 carry sequences related to transposons and homologous to the 24-nt siRNAs accumulated in wild-type plants that are the hallmarks of RNA-directed DNA methylation (RdDM). Analysis of a single-copy gene, SUPPRESSOR OF drm1 drm2 cmt3 (SDC), revealed that mom1 activates SDC with concomitant reduction of di-methylated histone H3 lysine 9 (H3K9me2) at the tandem repeats in the promoter region without changes in siRNA accumulation and cytosine methylation. The reduction of H3K9me2 is not observed in regions flanking the tandem repeats. The results suggest that MOM1 transduces RdDM signals to repressive histone modification in the core region of RdDM.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Factores de Transcripción
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Proteínas Nucleares
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Arabidopsis
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ARN de Planta
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Regulación de la Expresión Génica de las Plantas
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Metilación de ADN
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Silenciador del Gen
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Proteínas de Arabidopsis
Idioma:
En
Revista:
EMBO J
Año:
2010
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Reino Unido