Expression from baculovirus and serological reactivity of the nucleocapsid protein of dolphin morbillivirus.
Vet Microbiol
; 143(2-4): 384-8, 2010 Jul 14.
Article
en En
| MEDLINE
| ID: mdl-20005643
The nucleocapsid (N) protein of dolphin morbillivirus (DMV) was expressed from a baculovirus (Autographa californica nuclear polyhedrosis virus) vector and shown by SDS-PAGE and Western blot analysis to be about 57 kDa. Transmission electron microscopy revealed fully assembled nucleocapsid-like particles (NLPs) exhibiting the typical helical herringbone morphology. These NLPs were approximately 20-22 nm in diameter and varied in length from 50 to 100 nm. Purified DMV-N protein was used as antigen in an indirect ELISA (iELISA) and shown to react with rabbit and human antisera to measles virus (MV) and dog sera with antibodies to canine distemper virus (CDV). The iELISA was used for the demonstration of morbillivirus antibodies in the serum of cetaceans and manatees, showing potential as a serological tool for the mass screening of morbillivirus antibodies in marine mammals.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Baculoviridae
/
Morbillivirus
/
Proteínas de la Nucleocápside
/
Delfines
Tipo de estudio:
Screening_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Vet Microbiol
Año:
2010
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Países Bajos