A study was conducted to determine the quantitative effects of feeding amount and withdrawal period of corn distillers dried grains with solubles (DDGS) from the diet on growth performance, carcass quality, and pork fat fatty acid profile. A total of 432 pigs (29.8 +/- 0.2 kg of BW) were randomly allotted to 1 of 9 dietary treatments in a completely randomized arrangement. The 9 treatments were the control (D0), D15-0 wk, D15-3 wk, D15-6 wk, D15-9 wk, D30-0 wk, D30-3 wk, D30-6 wk, and D30-9 wk, where D0, D15, and D30 indicate the dietary content of DDGS (0, 15, and 30%, respectively) and 0 wk, 3 wk, 6 wk, and 9 wk indicate the withdrawal period of DDGS from the diets before slaughter (0, 3, 6, and 9 wk, respectively). A dietary DDGS inclusion rate of 15 or 30%, without or with a withdrawal period, had no effect (P = 0.76) on ADG, ADFI, and G:F, except for a slight reduction (0.87 vs. 0.92 kg/d; P < 0.05) in ADG when pigs received the D30-0 wk treatment compared with the D0 treatment. Carcass quality, LM quality, and Japanese fat color scores for backfat and belly fat were not affected by dietary DDGS content (backfat, P = 0.47; belly fat, P = 0.17) or withdrawal period (backfat, P = 0.33; belly fat, P = 0.95). Compared with pigs fed the D0 diet, a smaller belly firmness score was observed (P = 0.04) in pigs that received the D30-0 wk treatment, but belly firmness in pigs fed the other treatments was not different (P = 0.26) from that of pigs fed the D0 diet. Linoleic acid content (C18:2; P < 0.001) and iodine value (IV; P < 0.001) of belly fat increased with increasing dietary DDGS content. Withdrawal of DDGS from the diet for 0 to 9 wk before slaughter resulted in a linear reduction in C18:2 content and the IV of belly fat in pigs fed the D15 diets (C18:2 content: 14.6, 13.3, 12.6, and 10.9%; P = 0.001; IV: 67.3, 64.4, 64.1, and 62.7; P = 0.02; for 0-, 3-, 6-, and 9-wk withdrawal, respectively) and the D30 diets (C18:2 content: 17.3, 16.1, 14.2, and 12.4%; P < 0.001; IV: 71.2, 68.2, 64.5, and 62.7; P < 0.001; for 0-, 3-, 6-, and 9-wk withdrawal, respectively). These results indicate that an inclusion rate of DDGS up to 30% in grower-finisher diets has minor effects on growth performance and that the desired effect of reducing the C18:2 content and IV of pork fat could be elicited in as little as 3 wk after withdrawing DDGS from the diet before slaughter.