[Determination of adenosine in food by high performance anion exchange chromatography].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 40(5): 909-11, 917, 2009 Sep.
Article
en Zh
| MEDLINE
| ID: mdl-19950612
OBJECTIVE: To establish a high performance anion chromatography method for the determination of adenosine in food. METHODS: Adenosine in the food sample was extracted with Acetonitrile-water, and separated with Amino PAC PA10 Amino acid-separation column, with 0.25 mol/L NaOH as the mobile phase. A pulse ampere detector with Au as working electrode was used throughout the experiment. RESULTS: A good linear result was produced for adenosine in the range of 1.00-20.00 microg/mL. The inner-day RSD of peak area and retention time for the standard adenosine solution were 4.97% and 0.47%, respectively. The intra-day RSD of peak area and retention time for the standard adenosine solution were 6.08% and 0.52%, respectively. The proposed method was applied to food and satisfactory results were obtained, with and average recoveries of 93.3%-106.7% and 8.86% of RSD. CONCLUSION: The high performance anion exchange chromatography method is a simple, accurate, and reliable method for measuring adenosine in food, which can be completed within 10 minutes.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Adenosina
/
Cromatografía Líquida de Alta Presión
/
Aditivos Alimentarios
Idioma:
Zh
Revista:
Sichuan Da Xue Xue Bao Yi Xue Ban
Año:
2009
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
China