De novo sequencing of peptides on single resin beads by MALDI-FTICR tandem mass spectrometry.
J Am Soc Mass Spectrom
; 21(2): 215-9, 2010 Feb.
Article
en En
| MEDLINE
| ID: mdl-19914846
An efficient approach in combinatorial chemistry is the synthesis of one-bead-one-compound peptide libraries. In contrast to synthesis and functional screening, which is performed in a largely automated manner, structure determination has been frequently laborious and time-consuming. Here we report an approach for de novo sequencing of peptides on single beads by matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance (MALDI-FTICR) tandem mass spectrometry, using a resin with a photolinker for solid-phase peptide synthesis. Upon sorting out single beads, an efficient sample preparation on the MALDI target was developed that enables fragmentation upon irradiation of the bead-matrix mixture with the ultraviolet (UV)-MALDI laser, with enhanced yield of sequence-specific fragment ions at increased laser energy. This approach is illustrated by sequence determinations of two peptides from a library with sequences varying in a single amino acid; the feasibility with tandem-MS procedures and fragment ion assignment was ascertained by sustained off-resonance irradiation/collision induced dissociation (SORI/CID) and infrared multiphoton dissociation (IRMPD) fragmentation.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Péptidos
/
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
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Análisis de Secuencia de Proteína
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Espectrometría de Masas en Tándem
/
Análisis de Fourier
Idioma:
En
Revista:
J Am Soc Mass Spectrom
Año:
2010
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Estados Unidos