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A novel mechanism of sugar selection utilized by a human X-family DNA polymerase.
Brown, Jessica A; Fiala, Kevin A; Fowler, Jason D; Sherrer, Shanen M; Newmister, Sean A; Duym, Wade W; Suo, Zucai.
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  • Brown JA; Department of Biochemistry, The Ohio State University, Columbus, OH 43210, USA.
J Mol Biol ; 395(2): 282-90, 2010 Jan 15.
Article en En | MEDLINE | ID: mdl-19900463
During DNA synthesis, most DNA polymerases and reverse transcriptases select against ribonucleotides via a steric clash between the ribose 2'-hydroxyl group and the bulky side chain of an active-site residue. In this study, we demonstrated that human DNA polymerase lambda used a novel sugar selection mechanism to discriminate against ribonucleotides, whereby the ribose 2'-hydroxyl group was excluded mostly by a backbone segment and slightly by the side chain of Y505. Such steric clash was further demonstrated to be dependent on the size and orientation of the substituent covalently attached at the ribonucleotide C2'-position.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN Polimerasa beta Límite: Humans Idioma: En Revista: J Mol Biol Año: 2010 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN Polimerasa beta Límite: Humans Idioma: En Revista: J Mol Biol Año: 2010 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos