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Loss of heterozygosity at BRCA1/2 loci in hereditary and sporadic ovarian cancers.
Brozek, I; Ochman, K; Debniak, J; Morzuch, L; Ratajska, M; Stepnowska, M; Stukan, M; Emerich, J; Limon, J.
Afiliación
  • Brozek I; Department of Biology and Genetics, Medical University of Gdansk, Poland.
J Appl Genet ; 50(4): 379-84, 2009.
Article en En | MEDLINE | ID: mdl-19875889
Loss of heterozygosity at BRCA1/2 loci in breast and ovarian tumors is a suggested risk factor for germline BRCA1/2 mutation status. We evaluated the presence of losses of selected microsatellite markers localized on chromosomes 17 and 13q in hereditary and sporadic ovarian tumors. 151 consecutive primary ovarian tumors (including 21 with BRCA1/2 mutations and 130 without the mutations) were screened for loss of heterozygosity at loci on chromosomes 17 and 13q. Losses of heterozygosity of at least one microsatellite marker localized on chromosomes 17 and 13q were revealed in 123 (81.5%) and 104 (68.9%) tumors, respectively. Losses of all informative markers on chromosomes 17 and 13 occurred in 30 (19.9%) and 31 (20.5%) tumors, respectively. There was no difference in the frequency of losses at BRCA1 intragenic markers (D17S855 and D17S1323) between BRCA1-positive and BRCA1-negative patients. The frequency of losses on chromosome 17 was higher in high-grade than in low-grade carcinomas. Loss of heterozygosity on chromosomes 17 and 13q is a frequent phenomenon in both hereditary and sporadic ovarian cancers. The frequency of losses at BRCA1 intragenic markers in the ovarian tumor tissue is not strongly related to the presence of BRCA1 germline mutations.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Ováricas / Genes BRCA1 / Pérdida de Heterocigocidad / Genes BRCA2 Tipo de estudio: Etiology_studies / Risk_factors_studies Límite: Female / Humans País/Región como asunto: Europa Idioma: En Revista: J Appl Genet Asunto de la revista: GENETICA Año: 2009 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Ováricas / Genes BRCA1 / Pérdida de Heterocigocidad / Genes BRCA2 Tipo de estudio: Etiology_studies / Risk_factors_studies Límite: Female / Humans País/Región como asunto: Europa Idioma: En Revista: J Appl Genet Asunto de la revista: GENETICA Año: 2009 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Reino Unido