Combinatorial interaction between two human serotonin transporter gene variable number tandem repeats and their regulation by CTCF.
J Neurochem
; 112(1): 296-306, 2010 Jan.
Article
en En
| MEDLINE
| ID: mdl-19860858
Two distinct variable number tandem repeats (VNTRs) within the human serotonin transporter gene (SLC6A4) have been implicated as predisposing factors for CNS disorders. The linked polymorphic region in the 5'-promoter exists as short (s) and long (l) alleles of a 22 or 23 bp elements. The second within intron 2 (Stin2) exists as three variants containing 9, 10 or 12 copies of a 16 or 17 bp element. These VNTRs, individually or in combination, supported differential reporter gene expression in rat neonate prefrontal cortical cultures. The level of reporter gene activity from the dual VNTR constructs indicated combinatorial action between the two domains. Chromatin immunoprecipitation demonstrated that both these VNTR domains can bind the CCCTC-binding factor and this correlated with the ability of exogenously supplied CCCTC-binding factor to modulate the expression supported by these reporter gene constructs. We suggest that the potential for interaction between multiple polymorphic domains should be incorporated into genetic association studies.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Represoras
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Variación Genética
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Repeticiones de Minisatélite
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Proteínas de Transporte de Serotonina en la Membrana Plasmática
Límite:
Animals
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Humans
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Male
Idioma:
En
Revista:
J Neurochem
Año:
2010
Tipo del documento:
Article
Pais de publicación:
Reino Unido