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[125I]deoxycytidine used in a rapid, sensitive, and specific assay for herpes simplex virus type 1 thymidine kinase.
J Virol ; 24(1): 314-8, 1977 Oct.
Article en En | MEDLINE | ID: mdl-198581
[125I]deoxycytidine was a good substrate for herpes simplex virus type 1 thymidine kinase (TK), whereas [125I]deoxycytidine was a very poor substrate for cellular TK. Simple, sensitive, and specific assays for viral TK could be carried out in vivo and in vitro even in the presence of cell TK. Autoradiographic detection of incorporated [125I]deoxycytidine provided a rapid and simple method for detection of and screening for viral TK in infected as well as viral TK-transformed cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Timidina Quinasa / Simplexvirus / Desoxicitidina Tipo de estudio: Diagnostic_studies Idioma: En Revista: J Virol Año: 1977 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Timidina Quinasa / Simplexvirus / Desoxicitidina Tipo de estudio: Diagnostic_studies Idioma: En Revista: J Virol Año: 1977 Tipo del documento: Article Pais de publicación: Estados Unidos