An immunological method for quantifying antibacterial activity in Salmo salar (Linnaeus, 1758) skin mucus.

Narvaez, Edgar; Berendsen, Jorge; Guzmán, Fanny; Gallardo, José A; Mercado, Luis

Narvaez, Edgar; Berendsen, Jorge; Guzmán, Fanny; Gallardo, José A; Mercado, Luis.

Afiliación

Narvaez E; Laboratorio de Genética e Inmunologìa Molecular, Instituto de Biologìa, Facultad de Ciencias, Pontificia Universidad Católica de Valparaìso, Valparaìso, Chile.

Fish Shellfish Immunol ; 28(1): 235-9, 2010 Jan.

Article en En | MEDLINE | ID: mdl-19835960

RESUMEN

Antimicrobial peptides (AMPs) are a pivotal component of innate immunity in lower vertebrates. The aim of this study was to develop an immunological method for quantifying AMPs in Salmo salar skin mucus. A known antimicrobial peptide derived from histone H1 previously purified and described from S. salar skin mucus (SAMP H1) was chemically synthesized and used to obtain antibodies for the quantification of the molecule via ELISA. Using skin mucus samples, a correlation of bacterial growth inhibition versus SAMP H1 concentration (ELISA) was established. The results provide the first evidence for quantifying the presence of active AMPs in the skin mucus of S. salar through the use of an immunological method.

Asunto(s)

Salmo salar/inmunología; Animales; Anticuerpos Antibacterianos/inmunología; Ensayo de Inmunoadsorción Enzimática; Enfermedades de los Peces/inmunología; Enfermedades de los Peces/microbiología; Membrana Mucosa/inmunología; Salmo salar/microbiología; Piel/inmunología

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Salmo salar Límite: Animals Idioma: En Revista: Fish Shellfish Immunol Asunto de la revista: BIOLOGIA / MEDICINA VETERINARIA Año: 2010 Tipo del documento: Article País de afiliación: Chile Pais de publicación: Reino Unido

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