[Effects of hydrogen sulfide on the K ATP current in isolated rat ventricular myocytes].
Zhonghua Xin Xue Guan Bing Za Zhi
; 37(5): 445-8, 2009 May.
Article
en Zh
| MEDLINE
| ID: mdl-19781223
OBJECTIVE: To investigate the effects of endogenous and exogenous hydrogen sulfide (H(2)S) on the K(ATP) current in isolated rat ventricular myocytes. METHODS: Ventricular myocytes were isolated from rat heart by modified Langendorff perfusion with collagenase. K(ATP) current of single rat ventricular myocytes was recorded by whole-cell patch-clamp technique. RESULTS: The density of K(ATP) current was significantly reduced by 200 micromol/L DL-propargylglycine (PPG, an irreversible inhibitor of the H(2)S) [(5.3258 +/- 0.7556) pA/pF vs. (3.7856 +/- 0.4312) pA/pF, P < 0.01] in a time-dependent way. The density of K(ATP) current could be significantly increased by NaHS (a H(2)S donor, 9.375, 18.75, 37.5, 75, 150 micromol/L) in a concentration-dependent manner [(6.6310 +/- 0.6092) pA/pF vs. (9.0949 +/- 1.0259) pA/pF at 150 micromol/L, P < 0.01]. CONCLUSION: Both endogenous and exogenous H(2)S could open K(ATP) channels and enhance the K(ATP) current in rat ventricular myocytes.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Miocitos Cardíacos
/
Canales KATP
/
Sulfuro de Hidrógeno
Límite:
Animals
Idioma:
Zh
Revista:
Zhonghua Xin Xue Guan Bing Za Zhi
Año:
2009
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
China