Application of the high-performance liquid chromatography method with coulometric detection for determination of vitamin B(6) in human plasma and serum.
J Chromatogr B Analyt Technol Biomed Life Sci
; 877(27): 3151-8, 2009 Oct 01.
Article
en En
| MEDLINE
| ID: mdl-19733133
A reversed-phase high-performance liquid chromatography method (HPLC) with coulometric electrochemical detection has been developed and validated for the simultaneous analysis of pyridoxamine-5'-phosphate (PMP), pyridoxamine (PM), pyridoxal 5'-phosphate (PLP), pyridoxal (PL), pyridoxine (PN) and 4-pyridoxic acid (4-PA) in human plasma and serum. The isocratic separation was achieved on C(18) column (250mmx4.6mm, I.D., 5microm) with a mobile phase consisted of 35mM sodium phosphate containing 2.5mM heptanesulfonic acid, adjusted to pH 3.2 with 85% orthophosphoric acid and 12% methanol (v/v). Within-run and between-run precisions expressed by the relative standard deviations were less than 2.7% and 7.7% for all the analysed vitamins and 4-PA, respectively. The limits of detection (LOD) and quantification (LOQ) were: 0.8 and 2.6nM, 1.1 and 3.8nM, 1.5 and 4.5nM, 1.3 and 4.2nM, 1.1 and 3.7nM, 2.1 and 6.3nM for PMP, PM, PLP, PL, PN and 4-PA, respectively. The recoveries ranged from 90.4% to 98.4%. Stability of vitamins was checked under a variety of storage conditions. The developed application demonstrated acceptable sensitivity, precision, accuracy, stability, and linearity over the physiological concentration range. The major advantage of the proposed method is its great simplicity.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cromatografía Líquida de Alta Presión
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Vitamina B 6
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Técnicas Electroquímicas
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Adult
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Female
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Humans
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Male
Idioma:
En
Revista:
J Chromatogr B Analyt Technol Biomed Life Sci
Asunto de la revista:
ENGENHARIA BIOMEDICA
Año:
2009
Tipo del documento:
Article
País de afiliación:
Polonia
Pais de publicación:
Países Bajos