Incidence of chromosomal mosaicism in morphologically normal nonhuman primate preimplantation embryos.
Fertil Steril
; 93(8): 2545-50, 2010 May 15.
Article
en En
| MEDLINE
| ID: mdl-19732891
OBJECTIVE: To establish the exact rates of chromosomal mosaicism in morphologically normal rhesus macaque embryos by determining the chromosomal complement of all blastomeres. DESIGN: Retrospective rhesus monkey IVF study. SETTING: Academic laboratory and primate research center. PATIENT(S): Young fertile rhesus macaque females. INTERVENTION(S): Morphologically normal in vitro-produced rhesus macaque embryos were dissociated and cytogenetically assessed using a five-color fluorescent in situ hybridization assay developed for rhesus macaque chromosomes homologous to human chromosomes 13, 16, 18, X, and Y. MAIN OUTCOME MEASURE(S): The incidence and extent of chromosomal mosaicism in rhesus macaque preimplantation embryos. RESULT(S): Seventy-seven preimplantation embryos, displaying normal morphology and development, from 17 young rhesus macaque females were analyzed. Overall, 39 embryos (50.6%) were normal, 14 embryos (18.2%) were completely abnormal, and 24 embryos (31.2%) were mosaic. Of the 226 blastomeres analyzed in the mosaic group, 110 blastomeres (48.7%) were normal. CONCLUSION(S): The observed rate of mosaicism in good-quality rhesus embryos resembles previously documented frequencies in poor-quality human preimplantation embryos. A high incidence of mosaicism may limit the diagnostic accuracy of preimplantation genetic diagnosis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Blastocisto
/
Macaca mulatta
/
Mosaicismo
Tipo de estudio:
Diagnostic_studies
/
Incidence_studies
/
Observational_studies
/
Risk_factors_studies
Límite:
Animals
/
Pregnancy
Idioma:
En
Revista:
Fertil Steril
Año:
2010
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos