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Primary anti-D immunization by DEL red blood cells.
Kim, Kyeong-Hee; Kim, Kyung-Eun; Woo, Kwang-Sook; Han, Jin-Yeong; Kim, Jeong-Man; Park, Kyoung Un.
Afiliación
  • Kim KH; Department of Laboratory Medicine, Dong-A University College of Medicine, Busan, Korea. progreen@dau.ac.kr
Korean J Lab Med ; 29(4): 361-5, 2009 Aug.
Article en En | MEDLINE | ID: mdl-19726900
Extremely weak D variants called DEL are serologically detectable only by adsorption-elution techniques. A nucleotide change of exon 9 in RHD gene, RHD (K409K, 1227G>A) allelic variant is present in almost all the DEL individuals of East Asians. No DEL phenotype has yet been shown to induce a primary alloanti-D immunization in East Asia. A 68-yr-old D-negative Korean man was negative for anti-D at admission, and he developed alloanti-D after transfusion of red blood cells (RBC) from 4 apparently D-negative donors. Four donors who typed D-negative by routine serologic test were analyzed by real-time PCR for RHD gene and RHD (K409K). One donor was found to have RHD (K409K). This is the first case in which DEL RBCs with RHD (K409K) induced a primary alloanti-D immunization in Asian population. Because the DEL phenotype can induce an anti-D immunization in D-negative recipients, further discussion is needed whether RhD negative donors should be screened by molecular method and what an efficient genotyping method is for detecting the RHD gene carriers in Korea.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sistema del Grupo Sanguíneo Rh-Hr / Reacción a la Transfusión / Isoanticuerpos Límite: Aged / Humans / Male Idioma: En Revista: Korean J Lab Med Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2009 Tipo del documento: Article Pais de publicación: Corea del Sur

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sistema del Grupo Sanguíneo Rh-Hr / Reacción a la Transfusión / Isoanticuerpos Límite: Aged / Humans / Male Idioma: En Revista: Korean J Lab Med Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2009 Tipo del documento: Article Pais de publicación: Corea del Sur