Liquid chromatographic analysis of Hg(II) binding by thiol-rich peptides using both UV-vis and electrochemical detection.
J Chromatogr A
; 1216(39): 6752-7, 2009 Sep 25.
Article
en En
| MEDLINE
| ID: mdl-19700167
An existing method for HPLC determination of thiol-containing peptides has been successfully adapted to the analysis of mixtures of glutathione (GSH) and some related peptides with their Hg(II) complexes as a first approach to the study of phytochelatin extracts. The separation was achieved in a C18 column with a mobile phase of 0.1% trifluoroacetic acid (TFA) and 0.1% TFA/acetonitrile. Non-derivative UV-vis detection at 202 nm used in the original method has been complemented with amperometric detection at 1.2 V on glassy carbon electrode. Two different Hg(II)-GSH complexes were observed by both detection modes and confirmed by mass spectrometry.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cromatografía Liquida
/
Fitoquelatinas
/
Mercurio
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Idioma:
En
Revista:
J Chromatogr A
Año:
2009
Tipo del documento:
Article
País de afiliación:
España
Pais de publicación:
Países Bajos