Real-time PCR and enrichment culture for sensitive detection and enumeration of Escherichia coli.
J Microbiol Methods
; 79(1): 124-7, 2009 Oct.
Article
en En
| MEDLINE
| ID: mdl-19679150
Rapid enumeration of Escherichia coli strains by quantitative real-time PCR targeting the uidA gene was developed and confirmed for minced beef, tuna and raw oyster. Higher sensitivity (1 CFU/g of E. coli in all three food samples) was obtained by incubating for 7 h in TSB. Colony-directed E. coli specific TaqMan PCR assay could effectively distinguish colonies grown on various selective media within 1.5-h. Inspection of E. coli in food testing laboratories is important, and our rapid E. coli detection strategy will contribute to quality control in food industries.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Recuento de Colonia Microbiana
/
Reacción en Cadena de la Polimerasa
/
Escherichia coli
/
Microbiología de Alimentos
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Microbiol Methods
Año:
2009
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Países Bajos