Your browser doesn't support javascript.
loading
Bone repair by transplantation of hTERT-immortalized human mesenchymal stem cells in mice.
Nakahara, Hiroyuki; Misawa, Haruo; Hayashi, Takahiro; Kondo, Eisaku; Yuasa, Takeshi; Kubota, Yasuhiro; Seita, Masayuki; Kawamoto, Hironobu; Hassan, Wael A R A; Hassan, Reham A R A; Javed, Shahid M; Tanaka, Masato; Endo, Hirosuke; Noguchi, Hirofumi; Matsumoto, Shinichi; Takata, Katsuyoshi; Tashiro, Yuichi; Nakaji, Shuhei; Ozaki, Toshifumi; Kobayashi, Naoya.
Afiliación
  • Nakahara H; Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
Transplantation ; 88(3): 346-53, 2009 Aug 15.
Article en En | MEDLINE | ID: mdl-19667936
BACKGROUND: Human mesenchymal stem cells (hMSCs) are multipotent stem cells found in the adult bone marrow that have the capacity to differentiate into various mesenchymal cell types. The hMSCs may provide a potential therapy to restore damaged tissues or organs of mesenchymal origin; however, a drawback is their limited life span in vitro. METHODS: We immortalized normal hMSCs with retrovirally transmitted human telomerase reverse transcriptase cDNA. One of the immortalized clones (YKNK-12) was established, and the biological characteristics were investigated in vitro and in vivo. RESULTS: YKNK-12 cells were capable of differentiating adipocytes, osetoblasts, and chondrocytes. Osteogenically differentiated YKNK-12 cells produced significant levels of growth factors BMP4, BMP6, FGF6, FGF7, transforming growth factor-beta1, and transforming growth factor-beta3.. Microcomputer tomography T and soft X-ray assays showed an excellent calvarial bone healing in mice after transplantation of osteogenically differentiated YKNK-12 cells. These cells expressed human-specific osteocalcin and increased the gene expression of runt-related transcription factor 2, alkaline phosphatase, osteocalcin, and osterix in the bone regenerating area. YKNK-12 cell transplant corrected the bone defect without inducing any adverse effects. CONCLUSIONS: We conclude that hMSCs immortalized by transduction with human telomerase reverse transcriptase may provide an unlimited source of cells for therapeutic use in bone regeneration.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Cráneo / Enfermedades Óseas / Regeneración Ósea / Telomerasa / Trasplante de Células Madre Mesenquimatosas / Células Madre Mesenquimatosas Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals / Female / Humans Idioma: En Revista: Transplantation Año: 2009 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Cráneo / Enfermedades Óseas / Regeneración Ósea / Telomerasa / Trasplante de Células Madre Mesenquimatosas / Células Madre Mesenquimatosas Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals / Female / Humans Idioma: En Revista: Transplantation Año: 2009 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos