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Chloroquine and sulphadoxine-pyrimethamine sensitivity of Plasmodium falciparum parasites in a Brazilian endemic area.
Gama, Bianca Ervatti; de Oliveira, Natália K Almeida; Zalis, Mariano G; de Souza, José Maria; Santos, Fátima; Daniel-Ribeiro, Cláudio Tadeu; Ferreira-da-Cruz, Maria de Fátima.
Afiliación
  • Gama BE; Laboratory of Malaria Research, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil. bgama@ioc.fiocruz.br
Malar J ; 8: 156, 2009 Jul 14.
Article en En | MEDLINE | ID: mdl-19602248
BACKGROUND: The goal of the present study was the characterization of Plasmodium falciparum genes associated to malaria drug resistance (pfcrt, pfdhfr and pfdhps), in samples from two Brazilian localities. METHODS: Parasites from 65 P. falciparum samples were genotyped using nested-PCR and direct DNA sequencing. RESULTS: Six resistant sulphadoxine-pyrimethamine (SP) pfdhfr genotypes and one haplotype associated to SP sensitivity were detected. For pfcrt gene, SVMNT chloroquine (CQ)-resistant genotype was detected as well as the CVMNK CQ-sensitive haplotype in the same sample from Paragominas, that showed a SP-sensitive genotype. CONCLUSION: This study is the first to document the sensitivity of P. falciparum parasites to CQ and SP in Brazilian field samples. The importance of these findings is discussed.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasmodium falciparum / Pirimetamina / Sulfadoxina / Resistencia a Medicamentos / Cloroquina / Malaria Falciparum / Antimaláricos Tipo de estudio: Diagnostic_studies Límite: Animals / Humans País/Región como asunto: America do sul / Brasil Idioma: En Revista: Malar J Asunto de la revista: MEDICINA TROPICAL Año: 2009 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasmodium falciparum / Pirimetamina / Sulfadoxina / Resistencia a Medicamentos / Cloroquina / Malaria Falciparum / Antimaláricos Tipo de estudio: Diagnostic_studies Límite: Animals / Humans País/Región como asunto: America do sul / Brasil Idioma: En Revista: Malar J Asunto de la revista: MEDICINA TROPICAL Año: 2009 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido