Fine structure of Calymmatobacterium granulomatis with particular reference to the surface structure.
Indian J Med Res
; 93: 225-31, 1991 Jul.
Article
en En
| MEDLINE
| ID: mdl-1959951
ABSTRACT
PIP: The morphological structure of Calymmatobacterium granulomatis, the organism responsible for donovanosis, was investigated in biopsy material from 15 patients. The use of human material was necessary because this organism is difficult to grow in artificial culture media. 1-10 organisms per cell from 1.5-2.5 mcm in size were observed singly or in clusters, most frequently in the vacuolated cytoplasm of macrophages in the upper portion of the dermis. Most notable was the limitation of these organisms by a complex cell envelope comprised of at least 2 layers--the corrugated cell wall and the cytoplasmic membrane. In most cases, the protoplasm was enclosed by more than 2 trilaminar membranes. Cytoplasm at the periphery contained ribosomes and fine membranes, while the nuclear materials tended to be located in the cell's center. The organism's surface was covered with multiple filamentous processes (fimbriae) and round vesicles (blebs). Unique to this study was the finding of electron dense polar bodies at poles. The basal portion of the fimbriae was more electron dense than the remaining portion. Also notable was the observation that fimbriae are attached to the inner membrane and do not arise from the cell wall. The blebs also appeared to be endogenous to the cell wall and could be seen detaching and moving away from the parent organism. Finally, a distinct layer of homogenous material was observed on light microscopy to surround the organism and is postulated to represent capsular material.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bacilos Gramnegativos Anaerobios Facultativos
Límite:
Humans
Idioma:
En
Revista:
Indian J Med Res
Año:
1991
Tipo del documento:
Article
Pais de publicación:
India